BSL | Agents | Special Practicesa | Primary Barrier and Personal Protective Equipmenta | Facilities (Secondary Barriers)a |
---|---|---|---|---|
1 | Well-characterized agents not known to consistently cause disease in immunocompetent adult humans and present minimal potential hazard to laboratory personnel and the environment. | Standard Microbiological Practices | No primary barriers required; protective laboratory clothing; protective face, eyewear, as needed | Laboratory doors; sink for handwashing; laboratory bench; windows fitted with screens; lighting adequate for all activities |
2 | Agents associated with human disease and pose moderate hazards to personnel and the environment | Limited access; occupational medical services including medical evaluation, surveillance, and treatment, as appropriate; all procedures that may generate an aerosol or splash conducted in a BSC; decontamination process needed for laboratory equipment | BSCs or other primary containment device used for manipulations of agents that may cause splashes or aerosols; protective laboratory clothing; other PPE, including respiratory protection, as needed | Self-closing doors; sink located near exit; windows sealed or fitted withscreens; autoclave available |
3 | Indigenous or exotic agents; may cause serious or potentially lethal disease through the inhalation route of exposure | Access limited to those with need to enter; viable material removed from laboratory in primary and secondary containers; opened only in BSL-3 or ABSL-3 laboratories; all procedures with infectious materials performed in a BSC | BSCs for all procedures with viable agents; solid front gowns, scrubs, or coveralls; two pairs of gloves, when appropriate; protective eyewear, respiratory protection, as needed | Physical separation fromaccess corridors; access throughtwo consecutive self-closing doors;hands-free sink near exit; windows aresealed; ducted air ventilation systemwith negative airflow into laboratory;autoclave available, preferably inlaboratory |
4 | Dangerous and exotic agents that pose high individual risk of aerosol-transmitted laboratory infections and life-threatening disease that are frequently fatal, for which there are no vaccines or treatments; and related agents with unknown risk of transmission | Clothing change before entry; daily inspections of essential containment and life support systems; all wastes decontaminated prior to removal from laboratory; shower on exit | BSCs for all procedures with viable agents; solid front gowns, scrubs, or coveralls;b gloves;b full-body, air-supplied, positive pressure suitc | Entry sequence; entry through airlock with airtight doors;c walls, floors, ceilings form sealed internal shell; dedicated, non-recirculating ventilation system required; double-door, pass-through autoclave required |
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories; 6th Edition