Autoclave training is required every three years for anyone using an autoclave. Register for the Autoclave Safety learning track in Ability LMS.
Collect in leak-proof, puncture resistant secondary container with lid. Lined with a biohazard bag that fits appropriately. Add a biohazard label on the lid and side of container.
When ¾ full or within 90 days, place biohazard bag into an autoclave tray, Leave bag open to allow steam to penetrate bag. Add ~ ½ cup of water to bag. Autoclave bag in tray at parameters on machine.
At Cycle end, wait for pressure to release completely prior to opening door. Wear correct PPE: lab coat, long pants, closed toed shoes, autoclave gloves, and eye protection. Open door slowly and stand back as steam is released. When cycle was not completed or there is an error, contact a repair person and notify EHS. EHS tests the autoclave after maintenance /repair and at least once per year unless USDA/APHIS permits require shorter frequency.
After autoclaving allow to cool slightly then place in black opaque bag for disposal into dumpster. Orange bag cannot show through black bag. Black bag must be tied closed.
State and Federal regulations require biohazardous waste to be secured, treated and disposed of as defined in the required Biohazardous Waste Management Plan (PDF).
Autoclave usage and biohazardous waste sterilization issues are found too often in the University’s autoclave rooms.
These pictures are the wrong way to handle waste.
Clothing required:
PPE required:
Testing of autoclaves is an annual to quarterly requirement depending on permits and/or research materials being utilized. In order for the autoclaves to be tested consistently, personnel closely associated with the departments using the autoclaves must participate in the testing process.
Each department has a designated contact person for all autoclaves associated with the Department. The designated contact person will receive communications from EHS on testing dates, certification memos, parameter signs, and instructions on running the certification test kits.