Biosafety cabinet certified annually (BSL-2)

The functional operation and integrity of each BSC should be certified to NSF Standard 49 at the time of installation and annually thereafter by qualified technicians. Certification includes tests for cabinet integrity, HEPA filter leaks, downflow velocity profile, face velocity, negative pressure/ventilation rate, air-flow smoke pattern, and alarms and interlocks. Optional tests for electrical leaks, lighting intensity, ultraviolet light intensity, noise level and vibration may also be conducted. Special training, skills and equipment are required to perform these tests. Annual certification is required for BSCs that are used for work with human pathogens, recombinant DNA or human derived materials (e.g., cell lines, blood, etc.).


  • NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules. April 2019. Section IV-B-7-d-(1), (2) and Appendix G.
  • Biosafety in Microbiological and Biomedical Laboratories. HHS Publication No. (CDC) 21- 1112, 5th Edition.
  • MSU Biosafety Manual
  • MSU Bloodborne Exposure Control Plan

Corrective actions:

Provisions to assure proper safety cabinet performance and air system operation must be verified. BSCs should be certified at least annually to assure correct performance. To request service or certification contact the EHS at 517-355-0153.